Precautions for Culturing Cells in Cell Factories

In the fields of biopharmaceuticals and vaccine production, cell factories have become an important tool due to their space-saving design and high cell harvest rates.

In the fields of biopharmaceuticals and vaccine production, cell factories have become an important tool due to their space-saving design and high cell harvest rates. However, cells are very sensitive to their environment, so the following points must be observed during operation:

Aseptic Technique: All operations must strictly adhere to aseptic procedures when culturing cells. Any form of contamination can lead to experimental failure or even fatal damage to the cells.

Preheating: Preheat the cell factory and culture medium to the cultivation temperature before starting the experiment. This step can accelerate cell adhesion and significantly increase cell harvest rates. Preheating is especially crucial for larger culture containers.

Gentle Handling: Handle the cell factory gently to avoid vigorous shaking that can create bubbles. Bubbles can cause the culture medium to flow from the upper layers to the lower layers, leading to uneven distribution of the medium and clumping of cells.

Avoid Spraying Alcohol or Disinfectant on the Vent Cap: Alcohol or disinfectant can wet the hydrophobic filter membrane, which can prevent proper ventilation, affect gas exchange, and disrupt pressure balance during operation.

Culturing cells is a precise and meticulous task where correct operating methods are crucial. These precautions for operating cell factories are key to ensuring the smooth progress of cell culture work. Paying special attention to and adhering to these guidelines will help ensure the healthy growth of cells, providing high-quality raw materials and products for the biopharmaceutical and vaccine production fields.