There is a wide variety of pipettes available for clinical, forensic, drug discovery, chemistry, or any other life science research and analysis protocol you have. For experiments that need reliable and accurate liquid handling during sample preparation, you need to select the right pipettes for your lab if you want reproducible results.
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Having the right equipment can make or break any experiment that requires liquid handling. Whether working with manual pipettes or an automated pipetting solution, accurate and repeatable results are the main goal for any researcher—and this often depends on the instruments you use.
In this guide, you'll learn about the latest pipettes available and how to select the right one for your experiment.
Choosing the right pipette for your lab will depend on the application involved. You'll need to consider the type of liquid, the sampling volumes, task complexity, and the throughput required for your experiments.
There are a few major parameters to consider before you can narrow down your options to the right instrument. These are:
Air-displacement pipettes work well with aqueous liquids, but when you're handling problematic liquids, you'll need a positive-displacement model. Some of the biggest sources of pipetting errors are simply due to not selecting the right pipette for the liquid handling application involved.
There are also some collective considerations for choosing the right pipettes for your lab. If you'll be doing manual pipetting, you should select an instrument with a lightweight, ergonomic design to give you the ability to pipette longer without fatigue. Additionally, you'll need to know if the device features a volume-locking mechanism, or is autoclavable. Depending on your applications, you can also choose pipettes with a fixed volume.
Below, we discuss some of the best pipette types to select for your lab.
A fixed volume pipette is adjusted and factory calibrated at a specific volume, and dispenses the same quantity of liquid every time you pipette. Our PIPETMAN® L Fixed air-displacement pipette comes in fifteen different models that cover a range of volumes between 1 μL and 5 mL. With a lightweight design and a fixed volume that eliminates the risk of any volume error, these are the ideal pipettes for clinical diagnostics, quality control, and routine testing applications even for lesser trained lab technicians. These pipettes allow the lab techs to work faster and more confidently without having to adjust or verify the volume setting of the pipette.
Other benefits of fixed volume pipettes are:
Positive-displacement technology, like that used in our MICROMAN® E pipettes, is ideal when working with infectious, viscous, foaming, hot, cold, or volatile liquids.
The disposable piston within the capillary piston (CP) tip is in direct contact with the sample. The CP technology thus removes any air cushion inside the pipette and ensures the volume accuracy is not affected by the fluid's properties. This also helps prevent contamination and protects the sample, the user, and the pipette.
A MICROMAN E pipette gives you:
Figure 1. Comparison of the air-displacement and positive-displacement pipettes. Panel A shows the working principle of air-displacement pipettes with the piston integrated into the lower part of the pipette and an air cushion between the sample and the piston. Panel B describes positive-displacement pipettes: the disposable piston is part of the capillary tip and there is no air cushion that could be affected by the properties of the sample.
A single or multichannel PIPETMAN® L features reduced pipetting and tip ejection forces, a lightweight and improved ergonomics for a better balance and more comfortable use of the pipette. The design helps you pipette for longer without suffering from fatigue that could introduce errors into the sampling process. With improved comfort, PIPETMAN L offers accuracy and repeatability levels greater than the ISO 8655 requirements and meets the demand of users who need comfortable, secure, and reliable pipetting activities.
PIPETMAN L variable volume pipettes are:
For repetitive manual pipetting tasks, a Bluetooth® -connected pipette may help bring greater traceability to the lab. The PIPETMAN® M Connected pipette interacts in real-time with the TRACKMAN® Connected pipetting system, making planning and executing pipetting protocols faster, easier, and more reliable. It also offers accuracy and precision in both standard and repetitive pipetting modes, which helps to reduce errors in the lab.
Other benefits include:
In situations where you need to fill 96- to 384-well microplates accurately and repeatedly, the PLATEMASTER® is easy to use and provides high-throughput to match your lab's needs. You can reduce error while increasing your throughput without compromising on accuracy. With the PLATEMASTER, you're able to fill 96-well microplates in as little as 20 seconds and a 384-well plate in less than a minute.
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The PLATEMASTER allows you to:
For maximum throughput, while maintaining accuracy and repeatability, a PIPETMAX® automated pipetting solution provides an efficient platform for many liquid handling protocols. Researchers at the University of Missouri can prepare samples for 2,000 RT-qPCR reactions every week using a PIPETMAX liquid handler.
A PIPETMAX bench-top liquid handling solution can:
At Gilson, we provide access to the instruments and consumables that you need to produce trusted results. Our liquid handling solutions ensure that you can achieve your desired level of throughput without compromising on your experiments' precision, accuracy, or repeatability.
For more than 50 years, we’ve been creating pipettes with accuracy, comfort, and reliability as a top priority. Today, we offer a variety of pipettes in a range of ergonomic designs and volume sizes that enhance your pipetting experience and help you achieve reproducible, reliable results.
SHOP PIPETTES
Lots of precision and concentration are needed while using pipettes in laboratories. Pipettes are usually used to perform reactions that involve chemical reagents. Working with chemicals always involves risks. Increased risk of hand and shoulder ailments is associated with long hours of using pipettes.
Safety factors such as the length and weight of the pipette, the force needed to operate it, and how it fits into the hand, have to be considered while using pipettes. Pipettes are commercially available in different makes and models. All of them have various features related to technical specification, ease of use, and price. Comfort and ease of use are influenced by the pipette features. This article focuses on the ergonomic aspects of pipette design and use.
To ensure safe, comfortable, and efficient pipettes, ergonomics has to be taken into account during the design and manufacture of pipettes. For repeated use of pipettes, reliability and accuracy are key factors. Users must be taken into account while designing a product, such as the type of tasks performed by users, the type of equipment used by them, as well as their working environment. The design of the pipette must permit a neutral position of the user’s wrist and hand. An excessive amount of force should not be required to operate the pipette, and any contact stresses incurred to the hand should be minimized.
Given below are some of the major ergonomic points to be considered when choosing a pipette. The following characteristics are essential for a good pipette: good grip and comfortable to hold; light in weight; no sharp ridges or edges; easy tip ejection and fitting; suitable for right- or left-handed use; and an easy-to-use plunger requiring minimal force.
Sometimes, pipetting is repeated by laboratory workers. Research has shown that repetitive pipetting may lead to upper limb problems. The work routine can be varied to lessen the adverse effects of excessive pipetting; tasks requiring varying postures and taking regular breaks can be introduced. For prolonged working, taking frequent breaks works better than taking a single, long break. The person can also switch between different tasks. The effects of repetition can also be reduced by employing a multipipette or an electronic pipette.
Pipettes should not need an excessive amount of force during operation; the plunger can be difficult to operate if more force has to be applied. A forceful action may also be needed for highly viscous liquids. Hence, it is preferable to design pipettes with a shorter plunger, as they require less effort. There should be a smooth movement of depressing the plunger; minimal effort should be applied to hold the pipette. The effort needed to grip the pipette can be reduced by having textured material or small ridges. However, using an electronic pipette is the best way to reduce the amount of force to be applied. However, since their weight is more, they may cause strain when held for longer periods, though the electronic versions require less operational effort.
Since many types of pipettes are available on the market, the chosen one must be easy to operate and enable safe and secure pipetting. The following guidelines will help in the selection of a suitable pipette:
INTEGRA provides innovative solutions for Liquid Handling and Media Preparation applications which serve the needs of their customers in research, diagnostics and quality control laboratories.
Their instruments and plastic consumables are developed and manufactured in Zizers, Switzerland and Hudson, NH USA. In order to remain close to their customers, they maintain a direct sales and support organization in North America, the UK, France and Germany, as well as a network of over 100 highly trained distribution partners worldwide.
In recent years they have focused on developing a new and technologically advanced range of handheld electronic pipettes which are simple to use and meet the ergonomic needs of their customers.
Today they are proud to offer the widest range of electronic pipettes in the market spanning a range from single channel pipettes up to 384 channel bench-top instruments.
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